WebIn this protocol, we highlight the use of puromycin, the antibiotic used with the standard pLKO.1 vector. Typically, 1-10 µg/mL are sufficient to kill most untransduced mammalian … Web400 to 500 µg/mL would be a good starting concentration for G418 slection. (see attached G418 protocol with cell line dose differences) Agreed with Dr Zhou, a kill curve would help.
NUCLEAR REPROGRAMMING FACTOR - EP3418297B1 - 专利顾如
Webincubated without SA, while G418 or HmB was added in a two-fold dilution series in the remaining wells. The 96-well plates with cells and G418 or HmB was incubated at 37 C for … WebG418 and Puromycin Kill Curves. G418 and puromycin can be used to generate stabally transfected cell lines. However, the concentratio of these antibiotics that are required is … south jersey fireworks 2022
pCMV-EGFP-BoxB-RNAmotif-BoxB (RaPID质粒)(D3040-1μg)
WebPlate cells according to normal passaging protocols for the cell line in use. The next day, replace the media with media containing serial dilutions of the antibiotic to be used during … Web中国微生物资源共享平台,微生物菌种保藏中心,泰斯拓生物提供菌株,atcc菌株,菌株,质粒载体,慢病毒，细菌，真菌,支原体,衣原体,dsmz,ccug,atcc,分子技术试验,相关的技术服务,咨询电话0574-87917803 WebThe Expression Negative Control Lentivirus (Puromycin) expresses the gene for puromycin N-acetyl-transferase, which confers resistance to puromycin (Figure 3). Figure 1. … teach in delaware