WebEF1a-driven, Cre dependent expression of the site-specific recombinase FlpO. These AAV preparations are suitable purity for injection into animals. Delivery Volume 100 µL Titer ≥ 7×10¹² vg/mL Pricing $350 USD for preparation of 100 µL virus + $30 USD for plasmid. Storage Store at -80℃. Thaw just before use and keep on ice. WebOct 23, 2024 · We present evidence that PV-Cre recombination is: (1) spatially regulated and lobule specific; (2) detected in granule cells in the external and internal granule cell layers arising from strong, but transient Pvalb expression in progenitors between E13-E15; and (3) delayed in a subset of inhibitory interneurons, asynchronous with PV protein …
WebApr 11, 2024 · Adenosine monophosphate-activated protein kinase (AMPK) is the major sensor and regulator of cellular-energy metabolism. 6 AMPK, which is a serine (Ser)/threonine ... To express phospho-mutant AMPKα1 specifically in MSCs, we crossed Prkaa1-T172D fl/fl mice with Prx1-Cre mice to generate conditionally AMPKα1 phospho … WebActivated cardiac fibroblasts (CF) are crucial players in the cardiac damage response; excess fibrosis, however, may result in myocardial stiffening and heart failure development. Inhibition of activated CF has been suggested as a therapeutic strategy in cardiac disease, but whether this truly improves cardiac function is unclear. Objective: bubble tea chocolate
Addgene: pAAV-hSyn-DIO-mCherry
WebCre toxicity can also occur when expression is high enough to affect cell physiology, or when Cre recognizes genomic sequences that resemble loxP sites. Cre can damage … WebApr 12, 2024 · We delivered chicken DF1 cells producing four viruses encoding P1 bacteriophage Cre recombinase, human H3.3K27M, murine platelet-derived growth factor B ... To investigate the functional role of the activated microglia, we measured the expression of mRNAs encoding a panel of proinflammatory cytokines that inhibit tumor growth, … Web283 rows · Cre may be expressed globally under a broadly active promoter like CAG, or expressed only in a subset of cells under a more specific promoter (e.g. Rho-Cre is expressed in the retina). Fluorescent Cre: The fusion of Cre to a fluorescent reporter … In a previous post from our Plasmids 101 series, we learned how the Cre-loxP … export table with r3trans