2024 Bulk rna-seq macrophages adipose tissue

Bulk rna-seq macrophages adipose tissue

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August undefined, 2024

WebApr 12, 2024 · Chronic non-healing wounds, a prevalent complication of diabetes, are associated with increased mortality in diabetic patients. Excessive accumulation of M1 … WebIn addition, we also performed bulk RNA and exome sequencing on the aforementioned ACP tumors to gain insight into their basic properties. The transcriptional signatures and activating mutations of CTNNB1 exhibited by these tumor tissues were consistent with the typical genomic pattern of ACP (fig. S1, C and D, and table S2) (12, 36 ...

RNA-Sequencing Reveals Gene Expression and Pathway

WebIntegrated analysis of single-cell RNA-seq and bulk RNA-seq unravels tumour heterogeneity plus M2-like tumour-associated macrophage infiltration and … WebAug 4, 2016 · The core macrophage program initiated in pMacs is rapidly diversified as expression of transcriptional regulators becomes tissue-specific in early macrophages. This process appears essential for macrophage specification and maintenance, as inactivation of Id3 impairs the development of liver macrophages and results in selective … ead64545802

RNA-seq Based Transcriptome Analysis Reveals The Cross-Talk of …

WebMay 6, 2024 · Bone marrow adipose tissue reflects the accrual of adipocytes embedded within a complex extracellular matrix along with … WebJul 12, 2024 · These annotations were also consistent with data from scRNA-seq of macrophages and neutrophils from human SAT (Tabula Sapiens et al., 2024; Figure S2 ... useful tool for the optimization of deconvolution algorithms used to determine proportions of constituent cell types in adipose tissue bulk RNA-seq, e.g., CIBERSORT (Glastonbury … WebApr 24, 2024 · We applied our method to 106 bulk RNA-seq subcutaneous adipose tissue samples collected from both lean and ... S. P. et al. Obesity is associated with macrophage accumulation in adipose tissue. J ... csharp list directory

Airway Macrophages Encompass Transcriptionally and Functionally ...

Tissue-resident macrophages promote extracellular …

WebAug 5, 2024 · Macrophages in atherosclerotic patients are notably plastic and heterogeneous. Single-cell RNA sequencing (Sc RNA-seq) can provide information about all the RNAs in individual cells, and it is used to identify cell subpopulations in atherosclerosis (AS) and reveal the heterogeneity of these cells. Recently, some findings … WebJun 28, 2024 · Results: The ChIP-seq and transcriptome analysis of mouse adipose tissues demonstrated that a series of genes were activated by the histone modification of … csharp list binarysearchWebJan 6, 2024 · We queried bulk RNA-Seq data from human DM and NDM adipose tissues ( 29) for gene sets that were unique to each ATM subtype, reasoning that a bias toward … c sharp linter

"WebImmune checkpoint therapy (ICT) is among the widely used treatments for breast cancer (BC), but most patients do not respond to ICT and the availability of the predictive … " - Bulk rna-seq macrophages adipose tissue

Bulk rna-seq macrophages adipose tissue

Accurate estimation of cell composition in bulk expression

WebJul 15, 2024 · The results of RNA-seq and weighted gene co-expression network analysis (WGCNA) of chick subcutaneous and abdominal adipose tissues showed that the function of chick subcutaneous tissue was related to immunoreaction, and macrophage could be the major immune infiltration cell type in chicken subcutaneous adipose tissue, which … WebJun 1, 2024 · For bulk RNA-seq data analysis, Lyve-1 + and Lyve-1 – macrophages were identified and sorted as CD45 + CD11b + F4/80 + Lyve-1 + and CD45 + CD11b + F4/80 + Lyve-1 –, respectively. Mammary …

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WebApr 8, 2024 · Secondly, we have only performed bulk RNA-seq for mRNAs in UCB cells. As we know, UCB cells consist of a heterogeneous population of cells. ... Fain JN (2006) Release of interleukins and other inflammatory cytokines by human adipose tissue is enhanced in obesity and primarily due to the nonfat cells. ... Bucala R (2010) …

WebOct 22, 2024 · Q8. How much data can I expect from an RNA-Seq analysis of a mammalian RNA sample ? A8. A transcription profile (relative gene expression) of a sample can … WebImmune checkpoint therapy (ICT) is among the widely used treatments for breast cancer (BC), but most patients do not respond to ICT and the availability of the predictive biomarkers is limited. Emerging evidence indicates that tissue-resident macrophages (RTMs) inhibit BC progression, suggesting that their presence may predict …

WebApr 12, 2024 · Aim Obesity is linked to cardiometabolic diseases, however non-obese individuals are also at risk for type 2 diabetes (T2D) and cardiovascular disease (CVD). White adipose tissue (WAT) is known to play a role in both T2D and CVD, but the contribution of WAT inflammatory status especially in non-obese patients with … WebAccumulation of adipose tissue macrophages in obesity has also been shown in human WAT, using a combination of histomorphometry and flow sorting 19,22. ... Calculation of pseudobulk datasets to estimate adipose innervation. Approximate bulk RNA-seq datasets (pseudobulk) were obtained for visceral sNuc-seq samples by summing the total …

WebTo clarify adipocyte-hypertrophy associated transcriptomic alterations, fat cell size was related to RNA-Seq data from white adipose tissue and size-separated adipocytes. The relationship between adipocyte size and …

WebJan 28, 2024 · To identify early changes in MS brain tissues, we here analyzed the transcriptional profile of NAWM tissues without apparent macrophage (either microglia … csharp linq sortWebMay 5, 2024 · This in silico approach mimics bulk RNA-seq for the cell populations, ... Interestingly, CX3CR1 was recently shown to mark a unique population of tissue-resident ductal macrophages and TAMs in the mouse mammary gland (Dawson et al, 2024), though it is not expressed by normal breast tissue macrophages. csharp list add multiple itemsWebOct 8, 2024 · The reprocessed data and the bulk RNA-seq data for the lung macrophage subpopulations from the same study are available in S5 Data. (A) Expression profiles in single cells for selected genes. Each column represents RNA from a single cell. Y axis shows expression in TPM, calculated using Kallisto. Only the first 6 cells expressed … csharp list addrangeWebOct 22, 2024 · Q8. How much data can I expect from an RNA-Seq analysis of a mammalian RNA sample ? A8. A transcription profile (relative gene expression) of a sample can typically be made from 50 million clusters of reads. For detection of splice variants or low-expressing genes, then up to 150 million clusters of reads may be desirable. References : 1. ead70-800WebSep 19, 2024 · Background: The plasma membrane provides a highly dynamic barrier for cancer cells to interact with their surrounding microenvironment. Membrane tension, a pivotal physical property of the plasma membrane, has attracted widespread attention since it plays a role in the progression of various cancers. This study aimed to identify a … ead 800942WebNov 25, 2024 · Cardiac tissue-sorted macrophages (CD45+CD11b+CD64+) from neonatal (P1–3) and adult (8 weeks) mouse heart 10 days following MI (by permanent coronary … ead 766WebAdipose tissue RNASeq reveals novel gene-nutrient interactions following n-3 PUFA supplementation and evoked inflammation in humans ... We analyzed adipose gene expression using RNA sequencing in the fenofibrate and omega-3 fatty acid modulation of endotoxemia (FFAME) trial of healthy individuals at three timepoints: before and after n-3 … c sharp list files